CELL SEPARATION - Dissertations.se


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Figure 1. Acoustophoresis has gained increasing attention as a gentle, non-contact, and high-throughput cell and particle separation technique. It is conveniently used to isolate and enrich particles that are greater than 2 μm; however, its use in manipulating particles smaller than 2 μm is limited. In this work, we present an alternative way of using acoustic forces to manipulate sub-micrometer This high separation efficiency demonstrated a low nonspecific depletion/loss of cancer cells from the center fraction due to the acoustophoresis. Along with high separation efficiency, the cancer cell recovery (input to output) displayed an average MCF-7 recovery of 89.8 ± 5.3% in the center fraction (Fig.

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79(14), 5117–5123 (2007) Google Scholar ACOUSTOPHORESIS -A SOUND APPROACH TO CHIP BASED CELL HANDLING – T. Laurell1,2 1 Dept. Measurement Science and Industrial Electrical Engineering Div. Nanobiotechnology, Lund University, Lund, SWEDEN 2 Dept. Biomedical Engineering, Dongguk University, Seoul, SOUTH KOREA ABSTRACT The basic principles of acoustic cell and particle manipulation in chip integrated acoustic standing … 2018-09-19 A novel method, free flow acoustophoresis (FFA), capable of continuous separation of mixed particle suspensions into multiple outlet fractions is presented. Acoustic forces are utilized to separate particles based on their size and density.

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TY - JOUR. T1 - Free flow acoustophoresis: Microfluidic-based mode of particle and cell separation. AU - Petersson, Filip. AU - Aberg, Lena.

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ACOUSTOPHORESIS SEPARATION OF BACTERIA FROM BLOOD CELLS FOR RAPID SEPSIS DIAGNOSTICS P. D. Ohlsson*, K. Petersson, P. Augustsson and T. Laurell Lund University, SWEDEN ABSTRACT We present a significantly improved acoustophoresis method to separate bacteria from red and white blood cells.

The acoustophoresis is also a useful technique for rapid and controlled manipulation of bioparticles without affecting their viability6,10– 14.
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This paper presents the use of acoustophoresis, an ultrasound based microfluidic separation technology, for label-free, gentle and continuous separation of mononuclear cells (MNCs) from diluted whole blood. 2019-01-01 · Acoustophoresis technique enables cell separation by utilizing the size dependency of acoustic radiation force inside the microchannel. The acoustic forces on the particle do not depend on properties such as pH, surface charge, or ionic strength.

The method is shown to be suitable for both biological and nonbiological suspended particles.
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Draper Secures FDA Contract to Develop Acoustic Separation

Acoustic separation. Free Flow Acoustophoresis (FFA) can be considered as one of the most efficient approaches for high-throughput separation of microparticles or cells from complex suspensions such as blood samples. 31,173,174 A dedicated review series has been written on the subject.

CELL SEPARATION - Dissertations.se

KEYWORDS: Acoustophoresis, acoustic standing wave, cell separation, cell handling INTRODUCTION The use of acoustic standing wave forces has in recent years become a viable strategy for advanced cell manipulation and separation (acoustophoresis).

Acoustic forces are utilized to separate particles based on their size and density. The method is shown to be suitable for both biological and nonbiological suspended particles. In this thesis, a microfluidic technique called acoustophoresis is evaluated for plasma generation as well as for separation of white blood cells from whole blood. By moving blood cells in an acoustic field from a standing half-wave, pure plasma was generated at 10 µl/min. The acoustophoresis separation device is a silicon/glass microchannel [7] having a trifurcation inlet and outlet, Fig. 1. A piezoceramic crystal actuates the separation channel at /2 resonance, creating a force on suspended cells directed to-wards the vertical center plane of the channel. Label-free neuroblastoma cell separation from hematopoietic progenitor cell products using acoustophoresis - towards cell processing of complex biological samples.